Doğal Arazi Koşullarında Turunçgil Klorotik Cücelik Virüsü Titresindeki Değişimlerin İzlenmesi

Öz

İlk olarak Türkiye’de yetiştirilen turunçgillerde tespit edilen Citrus chlorotic dwarf-associated virus (CCDaV), turunçgillerde son yıllarda ortaya çıkan hastalıklardan biridir. Virüsün moleküler tanısı ve karakterizasyonu son yıllarda yapılmış olsa da etiyolojisine ilişkin mevcut bilgiler oldukça sınırlıdır. Bu çalışmada, etmeninin etiyolojisi açısından CCDaV’nin farklı bitki dokularındaki varlığının ve virüs titre düzeyinin mevsimsel ve dikey değişimlerinin belirlenmesi amaçlanmıştır. Doğal olarak enfekteli bir limon ağacından dört mevsim boyunca farklı doku ve bitki kısımlarından alınan örneklerde CCDaV’nin varlığı ve titre değişimleri incelenmiştir. Konvansiyonel ve gerçek zamanlı PCR analizleri sonucunda CCDaV’nin yaprak, çiçek ve meyve dokularında bulunduğu tespit edilmiştir. Virüs titre düzeyinin farklı mevsimler ve yönler açısından değişimi incelendiğinde, istatistiksel olarak en yüksek titre seviyesinin ilkbahar aylarında ağacın kuzey yönünde olduğu belirlenmiştir. Bununla birlikte, konvansiyonel PCR yöntemi kullanılarak CCDaV’ye yönelik rutin sürvey çalışmalarının yılın herhangi bir zamanında ve ağacın herhangi bir yönünden alınan yaprak örnekleriyle de gerçekleştirilebileceği ortaya konmuştur.

Anahtar Kelimeler

• CCDaV
• Klonlama
• qPCR

Proje Numarası

TÜBİTAK TOVAG 117O548

Monitoring Changes in Citrus Chlorotic Dwarf-associated Virus Titer under Natural Field Conditions

Öz

The citrus chlorotic dwarf-associated virus (CCDaV), which was first detected in citrus grown in Turkey, is one of recently emerged disease of citrus. The molecular diagnostic and characterization of the virus was carried out in recent years. However, the information available on the etiology of the virus is extremely limited. This study aimed to determine the presence of CCDaV in different tissues, and seasonal and vertical changes of virus titter in terms of the etiology of the causal agent. The presence and changes in CCDaV titter were investigated from samples taken from different tissues and parts of a naturally infected with lemon tree throughout the four seasons. As a result of conventional and real-time PCR tests, CCDaV was detected in leaves, flowers and fruits. Analysis of titter changes of CCDaV in different seasons and directions revealed that the virus titter was statistically at the highest level in the northward direction in the spring months. However, it has been determined that routine CCDaV surveys can be performed from leaf samples at any direction of tree and time of the year with conventional PCR as well.

Anahtar Kelimeler

• CCDaV
• Cloning
• qPCR

Destekleyen Kurum

Scientific and Technological Research Council of Turkey (TÜBİTAK)

Proje Numarası

TÜBİTAK TOVAG 117O548

Etik Beyan

This article does not contain any studies with human participants or animal performed by any of the authors

Teşekkür

This research is part of the first author’s Ph.D. thesis, supported through a grant provided from the Scientific and Technological Research Council of Turkey (TUBITAK-TOVAG 117O548). We thank Bengi TOPKAYA for technical asistance and Fatih KAHRIMAN for his help with the statistical analysis.

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